WebWe examined the uptake and intracellular transport of the fluorescent glucosylceramide analogue N-[5-(5,7-dimethyl BODIPYTM)-1-pentanoyl]-glucosyl sphingosine (C5-DMB-GlcCer) in human skin fibroblasts, and we compared its behavior to that of the corresponding fluorescent analogues of sphingomyelin, galactosylceramide, and … WebProduct Description. BODIPY FL C5-HPC is a green-fluorescent probe for labeling lipid membrane. This probe has been used for investigations of the uptake of antineoplastic ether lipids and for the characterization of lipid domains by microscopy. It is relatively insensitive to environment and pH changes.
Bodipytm Fl C5 Spinghomyelin Thermo Fisher Bioz
WebAug 29, 2024 · The nucleotide sequence encoding C-terminal HIS-tagged human EL (NP_006024.1) was inserted into a mammalian expression vector containing a cytomegalovirus (CMV) promoter. EL protein expression was performed through transient transfection in HEK293 cells cultured in serum-free media. WebThe green fluorescent fatty acid, BODIPY™ FL C 5 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Unlike fluorophores like pyrene, DPH and … The green fluorescent fatty acid, BODIPY FL C5 can be used as a synthetic … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes digital academy scot gov
Characterization of a BODIPY-labeled fluorescent fatty acid ... - PubMed
WebBODIPY FL C5-HPC is a green-fluorescent probe for labeling lipid membrane. This probe has been used for investigations of the uptake of antineoplastic ether lipids and for the … WebBODIPY FL C5. BODIPY FL C5 is a green-fluorescent dye that is reactive with primary amines on biomolecules such as peptides, proteins, modified nucleotides and biopolymers. It is suitable for live-cell membrane experiments. This carboxylic acid may react with amines using a carbodiimide like EDAC or DIPC and to form ester-linked conjugates or ... WebThe BODIPY-labeled fatty acid analogues are a useful addition to the tools employed to study the cellular uptake and metabolism of lipids. In this study, we show that BODIPY FL C (16) binds to purified liver and intestinal fatty acid-binding proteins with high affinity at a site similar to that for the physiological fatty acid oleic acid. digital academics city of malabon university