Cyclin d1 clone sp4 flow cytometry
WebMay 3, 2012 · Primary Abs were cyclin D1 clone SP4 from NEOMarkers (Lab Vision), Ki-67 clone MIB1 (DakoCytomation), and p53 clone DO-7 (Novocastra), and for SOX11 the SOX11 Ab HPA000536 (Atlas Antibodies AB) was used with Bond primary Antibody Diluent (AR 9352; Vision Biosystems). WebFigure Legend Snippet: The SCP3–pAKT–cyclin D1–NANOG axis in tumor cells correlates with the stage and prognosis of cervical cancer. A, IHC staining of cyclin D1 was performed on a tissue microarray. High magnification images are shown in inset. Scale bar, 250 mm. CIN, cervival intraepithelial neoplasia; LG-CIN, low-grade CIN; HG-CIN, high-grade CIN.
Cyclin d1 clone sp4 flow cytometry
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WebJan 15, 2024 · Transfection efficiency was determined by flow cytometry using a plasmid encoding the enhanced green fluorescent protein eGFP (Amaxa Biosystems). ... Purified monoclonal antibodies to cyclin D1 (clone SP4, RM-910), cyclin D3 (clone DCS-22, MS-215-R7) all from NeoMarkers, Fremont, CA and cyclin D2 (10R-C149a) from Fitzgerald … WebSpecificity. The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions. Application. FC (QC tested) Application details. Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining. Reactivity.
WebSep 1, 2011 · In our series, cyclin D1 showed similar sensitivity (96%) to annexin-1 and aberrantly stained only 1 case of MZL (cytogenetic analysis of the splenectomy specimen in this case was negative for t (11;14) and lacked all of the morphologic and genetic characteristics of mantle cell lymphoma). WebFeb 1, 2001 · Cyclin D1 expression is used as a diagnostic marker for MCL and for differentiating, along with other parameters, between MCL and the other B-cell disorders. …
WebCCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. WebImmunogen Synthetic peptide from C-terminus of human cyclin D1. Form Liquid Concentration 0.046mg/mL Purification Antigen affinity chromatography Storage Buffer PBS, pH 7.6, with 1% BSA Contains 0.1% sodium azide Storage Conditions -20° C, Avoid Freeze/Thaw Cycles RRID AB_2537875 Applications Tested Dilution Publications Flow …
WebWe developed a flow cytometry method to detect cyclin D1 overexpression using the monoclonal antibody (MoAb) 5D4, and characterised its frequency in 93 B-cell …
WebClone G124-326 recognizes human Cyclin D1. It does not cross-react with human cyclins D2 and D3. Recombinant full-length human Cyclin D1 was used as immunogen. ... (Clone-specific: Flow cytometry). Gong J, Traganos F, Darzynkiewicz Z. Threshold expression of cyclin E but not D type cyclins characterizes normal and tumour cells entering S phase ... marshfield medical center billingWebConcentration Unitmg/mLConcentration1Quantity0.1 mgVolume0.1 mLImmunogenPurified cyclin D1 proteinBackground InformationCyclin D1 (PRAD1, Bcl-1) is a… Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL Immunogen Purified cyclin D1 protein Background… marshfield medical center erWebSep 11, 2015 · The primary antibodies, monoclonal rabbit anti-human cyclin D1 clone SP4 (Ready to Use DAKO), monoclonal rabbit anti-human CD5 clone SP19 (Ready to Use, DAKO), monoclonal mouse anti-human... marshfield medical center gift shopWebCyclin D1 is part of the cell cycle regulation and oncogenic transformation in mammalian cells. The cyclin-dependent kinases, CDK4 and CDK6, are associated with and activated by cyclin D1 thereby promoting G1 phase … marshfield medical center fax numberWebThe CCND1 gene encodes the cyclin D1 protein. The human CCND1 gene is located on the long arm of chromosome 11 (band 11q13). It is 13,388 base pairs long, and translates into 295 amino acids. [7] Cyclin D1 is … marshfield medical center jobshttp://www.cyto.purdue.edu/cdroms/cyto10a/researchcenters/media/darz/cyclins.pdf marshfield medical center er marshfield wimarshfield medical center iron mountain