Electroporation of phaeodactylum tricornutum
WebHerein reported are new tricyclic cytidine compounds, such as 8-diethylamino-tC (8-DEA-tC), that respond to DNA and/or RNA duplex formation with up to a 20-fold increase in fluore WebApr 1, 2024 · In this study, the correlation between the specific growth rate of Phaeodactylum tricornutum and fucoxanthin (fucoxanthin) biosynthesis was investigated both in batch and in semi-continuous cultures. Fucoxanthin content from P. tricornutum biomass showed a positive correlation with specific growth rate during the …
Electroporation of phaeodactylum tricornutum
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WebDec 4, 2012 · As concerns the classical microalgal transformation systems referred, Chlamydomonas and Phaeodactylum, there is a wide collection of strong constitutive homologous promoters, such as the rubisco small subunit RBCS2 promoter fused to the heat shock protein (HSP70A) promoter of C. reinhardtii or the fucoxanthin chlorophyll … WebApr 8, 2024 · Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. 13 September 2024 ... The electroporation parameters were set as follows ...
WebApr 7, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to... WebJun 1, 2012 · In a study using microalgae Phaeodactylum tricornutum, the pHY18 vector was ligated with gene of interest DGAT2 and then transformed by electroporation …
WebCurrently, training through the Biochemistry PhD program in Dr. Karas's lab, which focuses on developing innovative strategies for genome engineering. I study Phaeodactylum tricornutum, a eukaryotic micro-algae, and have created a platform for rapid organelle genome engineering in this model system. My research interests include: 1) organelle … WebJul 26, 2024 · Fucoxanthin amount has been shown to double in engineered P. tricornutum overexpressing the gene encoding lycopene-β-cyclase (LCYB) under the control of the fcpB promoter, when fed with basal...
WebKey words: diatom; electroporation; multi-pulse; Phaeodactylum tricornutum Diatoms are important primary producers in the ecosystem that have crucial roles in the global carbon … journal of nanoparticle research分区WebAug 27, 2024 · The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), … journal of natural fiberWebPhaeodactylum tricornutum (CCAP1055/1) Perkinsus marinus (ATCC PRA240) Oxyrrhis marina (CCMP 1788, CCMP 1795) Alexandrium catenella (CCMPBF-5) Karlodinium veneficum (CCMP1975) Amphidinium carterae (CCMP1314) Diplonema papillatum (ATCC 50162) Eutreptiella gymnastica (SCCAP K-0333) how to maintain gravel drivewayWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 journal of national black nurses\u0027 associationWebAug 20, 2024 · Phaeodactylum tricornutum is a model diatom with defined culture conditions, but routine genetic manipulations are hindered by a lack of simple and robust genetic tools. ... In contrast to ... journal of narrative researchWebAn efficient nuclear transformation method has been established for the pennate marine diatom Phaeodactylum tricornutum using an electroporation system that drives multisequence pulses to introduce foreign DNAs into the cells. By removing excess salts in the culture medium and optimizing pulse condi … journal of natural fibers loginWebGenetic transformation system is very important for both basic biological research and commercial exploitation of diatoms. Here we describe a high-efficiency nuclear … how to maintain green card status in the us