WebNov 12, 2015 · In addition, in response to DNA damage, p53 induced FTH1 and suppressed transferrin receptor, which regulates iron entry into cells. ... Primers used for amplification and mutagenesis are shown in ... WebFTH1 (a.k.a. FHC, FTH, FTHL6, HFE5, PIG15, PLIF) Promoter SFFV Tag / Fusion Protein. NLS-iLID-GFP (N terminal on insert) Cloning Information Cloning method Ligation Independent Cloning 5′ sequencing primer n/a
Regulation of iron homeostasis by the p53-ISCU pathway
WebFeb 1, 2024 · FTH1 expression is affected by promoter polymorphism and not DNA methylation in response to DHV-1 challenge in duck. Author links open overlay ... (RT-PCR) (Xu et al., 2014b) with primers shown in Table 1. The thirty ducklings were inoculated with 0.4 mL of allantoic liquid containing DHV-1 (ELD50 10 −4.6 /0.2 ml) according to our ... WebSpecificity. Amplicon sequence validated with next generation sequencing (NGS). Minimal primer dimer formation and genomic DNA cross reactivity. Amplification Efficiency. … shipyard apartments sackets harbor ny
Fth1 Mouse qPCR Template Standard (NM_010239) from OriGene …
WebMay 6, 2024 · This Primer reviews the mechanisms underlying ferroptosis, highlights connections to other areas of biol. and medicine, and recommends tools and guidelines for studying this emerging form of regulated cell death. ... Overexpression of FTH1 in PC-12 cells impaired ferritinophagy and downregulated microtubule-assocd. protein light chain 3 … WebFeb 1, 2024 · In conclusion, high expression of FTH1 promoted proliferation and inhibited apoptosis of leukemic cells through the ferroptosis pathway and is thus a potential risk factor that affects the prognosis of non-M3 AML in children. ... The primers were designed and synthesized by Bioengineering (Shanghai) Co., Ltd. and the sequences are shown in ... WebIn this study, 10 candidate reference genes (ZC3H10, FTL, LGALS1, RPL27, GAPDH, FTH1, DCN, TCTP, NDUS5, and UBIM) were initially tested for amplification efficiency … shipyard apprenticeship programs virginia