WebQ Sepharose Fast Flow is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein … WebHiTrap Q FF is a strong anion exchange chromatography column for small-scale protein purification. Packed with Q Sepharose Fast Flow strong anion exchange resin. Convenient …
CM Sepharose Fast Flow DEAE Sepharose Fast Flow Q Sepharose Fast Flow …
WebChelating Sepharose Fast Flow consists of iminodiacetic acid groups coupled to Sepharose 6 Fast Flow by stable ether linkages and sufficiently long spacer arms (Fig 2). The Sepharose 6 Fast Flow base matrix is a highly cross-linked, 6% agarose with excellent chemical and physical stability. The cross-linking of the matrix has been optimized to give Web凝胶过滤柱层析 介质：Sepharose 4 FF 超滤浓缩到适当体积 按照平衡柱床、丄样、洗脱程序层析 ①具有准确的转录后修饰功能 ,表达的糖基化药 物蛋白在分子结构、 理化特性和生物学功能方面最 接近于天然蛋白分子 ②具有产物胞外分泌功 能 ,便于下游产物分离纯化; ③具有重组基因的高效 扩增和表达能力; ④具有贴壁生长特性 ,且有较高的 耐受剪切力和渗透压能 … nafem trade show 2021
CM Sepharose Fast Flow DEAE Sepharose Fast Flow Q Sepharose …
Web弃上清收集包涵体并使用变性剂溶解；透析复性后，包涵体溶解液上清用0.2 μM滤膜过滤，Ni-chelating sepharose FF亲和层析柱纯化蛋白，用含100、500 mmol/L咪唑的Elution Buffer分别洗脱蛋白，收集洗脱流穿峰，最后用SephadexG-25 除盐柱脱盐处理，采用Bradford方法测定目的蛋白浓度。 WebQ-sepharose is a strong anion exchanger. In order to clean it, different aqueous solutions can be used. Some of them usually contain one of the following reactives: around 1.0 M NaOH, up to 8 M... WebANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross- linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins. Characteristics of the ion exchangers are listed in Table 2. nafe obituary