Thp 1 cell lines
WebBelow are a few tips we recommend to help get your THP1 cells growing: • These cells need to be passaged between 3 x 10 5 and 7 x 10 5 cells/ml (we recommend 5 x 10 5 cells/ml). … WebAll lanes : Anti-CD64 antibody [EPR4623] at 1/1000 dilution Lane 1 : Wild-type THP-1 cell lysate Lane 2 : FCGR1A knockout THP-1 cell lysate Lane 3 : U937 cell lysate Lane 4 : HEK-293 cell lysate Lysates/proteins at 20 µg per lane. Performed under reducing conditions. Observed band size: 45-50 kDa why is the actual band size different from the predicted? ...
Thp 1 cell lines
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WebAll lanes : Anti-SHP1 antibody [Y476] at 1/1000 dilution Lane 1 : Wild-type THP-1 cell lysate Lane 2 : PTPN6 knockout THP-1 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : K562 cell lysate Lysates/proteins at 20 µg per lane. Performed under reducing conditions. Predicted band size: 68 kDa Observed band size: 70 kDa why is the actual band size different from … WebAug 5, 2005 · We have identified human monocytic (THP-1) and myelogenous CD34 + (KG-1) leukemia cell lines that can be differentiated rapidly into mature dendritic cells (DCs) when cultured in serum-free medium containing GM-CSF, TNF-α, and ionomycin. These hematopoietic cell line-derived DCs are highly pure and monotypic, and display the …
WebCytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer 29:333-338(2000) DOI= … Web4. Incubate the culture at 37°C incubator with 5% CO 2. Cultures should be monitored daily. 5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for subculture for a number of days. Cells should be left as much as possible over this time and only subcultured when the cell density reaches 8x10 5 cells/mL.
WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM … Web4. Incubate the culture at 37°C incubator with 5% CO 2. Cultures should be monitored daily. 5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for …
WebTo be able to demonstrate this interaction in the system, experimental goals had to be switched, so, since the TLR-4/MAPK pathway is active in the THP-1 cell line, we assume that, perhaps, TLR-4 (previously reported in mice) actually initiates the activation of the MAPKs cascade, yielding the experimentally observed cell response.
WebThe THP-1 cell line is a human monocytic leukaemia cell line which was established in 1980 by Tsuchiya et al. . It was derived from the blood of a patient with acute monocytic … kosher catering manchesterWebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a temperature of 37 o C in a humidified incubator with … man just a chair short fiction wordpressWebMonociles and their macrophage and dendritic-cell progeny serve three main functions the the immune system. Those have phagocytosis, antigen showcase, and cytokine … kosher cbd edibleskosher catering pittsburghWeb3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at … manjusri secondary school cut off pointWebTo be able to demonstrate this interaction in the system, experimental goals had to be switched, so, since the TLR-4/MAPK pathway is active in the THP-1 cell line, we assume … kosher cbd productsWebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … kosher catering ottawa