2024 Top/fopflash reporter assay

Top/fopflash reporter assay

Author: bqzy

August undefined, 2024

Web目的. 研究VPS26在高脂环境中对大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSC）成骨、成脂分化作用的机制，探讨VPS26对高脂大鼠种植体骨结合和裸鼠异位成骨的影响。 Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a …

Cancers Free Full-Text THY1 (CD90) Maintains the Adherens …

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … building a floor plan online

Improving the response to oxaliplatin by targeting chemotherapy …

WebReporter Assays. A bioluminescent reporter assay consists of both a luciferase reporter enzyme and a detection reagent that provides the enzyme substrate. When the reporter enzyme and detection reagent are combined, the light emitted is proportional to reporter gene expression levels and is detected using a luminometer. Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 Web12. apr 2024 · TOP/FOP flash luciferase reporter assay was carried out to detect the activity of Wnt pathway. Besides, qPCR, RNA pulldown and luciferase reporter assays were conducted to probe into the target gene of miR-489-3p. Immunoprecipitation-western blot (IP-western blot) analysis was implemented to assess the effect of USP48 on the … building a flow chart in powerpoint

TCF/LEF Reporter Kit (Wnt Signaling Pathway) - BPS …

miR-489-3p promotes malignant progression of non-small cell

Web12. júl 2024 · RNA immunoprecipitation, RNA pull down and luciferase reporter assays were used to identify LUCAT1 and TCF7L2 as the direct target of miR-5582-3p. The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash reporter assays, western blot and immunohistochemistry (IHC). Results: WebA, B, C. Western blot analysis and TOP/FOP Flash reporter assays confirmed that TP73-AS1/miR-27b-3p/TMED5 axis affected wnt/β-catenin pathway. D. Representative images of the tumor lumps of each ... building a flowchart in wordWebPred 1 dňom · The reporter construct is introduced into a suitable cell line, and the potency of a specific analyte is determined by measuring the change in reporter gene expression in response to that analyte. Reporter gene assays (RGAs) are simpler, and more rapid and accurate compared to conventional cell-based assays that depend on phenotypic … building a flow chart microsoft office

"WebTo verify our hypothesis, we first used TOP/FOPFlash reporter assays to detect the effect of miR-504 on Wnt-β-catenin signaling pathway activity, and found that it was significantly reduced in ... " - Top/fopflash reporter assay

Top/fopflash reporter assay

miR-489-3p promotes malignant progression of non-small cell

WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF … WebDescription The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready …

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Web13. apr 2024 · Scheme of CRISPR/Cas-based and quantum dot nanobeads (QDNBs) labeled lateral flow assay (CQ-LFA). a Cas12a cleaves the VZV ORF31 and FAM-ssDNA-biotin reporters under the guidance of a single crRNA.b Reaction products were loaded onto the LFA. When the VZV DNA was absent, intact FAM-ssDNA-biotin reporters were captured by … Web2. apr 2024 · 胃癌细胞系MGC-803购自ATCC；SGC-7901细胞购自中国科学院细胞库；抗PRRX2、β-catenin、c-Myc、cyclin D1和GAPDH I抗以及HRP标记的山羊抗兔Ⅱ抗均购自ProteinTech；转染试剂脂质体Lipofectamine 3000(Invitrogen)；胰蛋白酶(索莱宝生物科技有限公司)；MTT细胞活力检测试剂盒(碧云天生物 ...

Web10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot assay showed that downstream targets... WebTop和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP值来表示 …

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay was used to analyze the activity of Wnt/β-catenin signaling pathway. The vector pRL-SV40 was served as the internal reference. Cells in the control groups were co-transfected with pRL-SV40 and TOP/FOP flash (Promega). Cells in NC groups were co-transfected with pRL-SV40, TOP/FOP flash and si … Web16. aug 2024 · TOP/FOP Flash assays were conducted to determine the effects of miR-504 on Wnt/β-catenin signaling. Results Analysis of TCGA transcriptomic data showed that low miR-504 expression correlated with ME subtype transition and poor survival in …

Web背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。

Web9. apr 2024 · TOP/FOPflash assay and qRT-PCR indicated that expression alterations of OCC-1D could change Wnt signaling activity in colon cancer cells. Consistently, … crowdpose数据集简介WebPred 1 dňom · A dual reporter assay is based on the simultaneous expression and measurement of two individual reporter enzymes within a single system. Typically, the primary or the experimental reporter is the marker of the gene or response element in question, whereas the activity of the co-transfected secondary or control reporter serves … crowdpowerpartners株式会社 engageWeb6. apr 2024 · The DNA was a mix of 2.25 μg TOP/FOP-Flash reporter and 0.25 μg pRL-TK . The Dual-Luciferase Reporter Assay system (Promega, E1910) was used for collection and luminescence measurement, according to manufacturer's instructions. In short, on day 3 or 4 of differentiation the cells were washed with PBS and 500 μl Passive Lysis Buffer … building a flower bed borderWebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … crowdpose数据集格式WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … building a flower bed with landscape timbersWeb12. apr 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ... building a floor for a sunroomWebNational Center for Biotechnology Information building a flower bed retaining wall